Journal: PLoS ONE

Article Title: Peptide Inhibitor of Complement C1 (PIC1) Rapidly Inhibits Complement Activation after Intravascular Injection in Rats

doi: 10.1371/journal.pone.0132446

Figure Lengend Snippet: Hemolytic assays using (A) Wistar rat serum, (B) mouse plasma and (C) cynomolgus monkey serum were performed with increasing amounts PA-dPEG24 dissolved in 100mM Na 2 HPO 4 with 0.9% NaCl buffer for rat serum, 10mM Na 2 HPO 4 with 0.9% NaCl for cynomolgus monkey serum and 0.9% NaCl for mouse plasma. Serum or plasma were incubated with peptide and then added to human AB erythrocytes. Values are the means of three independent experiments. Error bars represent the SEM.

Article Snippet: In a total volume of 375μL, 1x10 9 human AB erythrocytes (non-sensitized) were combined with various concentrations of PA-dPEG24 (dissolved in 0.1M Na 2 HPO 4 , 0.9% NaCl, pH 7.4) and 50μL of Wistar rat serum (Innovative Research, Inc.) for 15min at 37°C.

Techniques: Incubation

Journal: Biology of Sex Differences

Article Title: Sexual dimorphism in myocardial acylcarnitine and triglyceride metabolism

doi: 10.1186/s13293-016-0077-7

Figure Lengend Snippet: Sex differences in expression profile of genes involved in lipid metabolism. a Number of genes modulated between NOD and Wistar rats by sex from ANOVA model. A section sign (§) indicates that pathways differ between sexes by the rank-sum test ( P ≤ 0.05). b 3D score plot of first three principal components (PCs) for genes from principal component analysis. c Shows the radar plot of loading factors of PCs for gene analysis. The purple line and circles represent PC1, the brown line and circles represent PC2, and green line and circles represent PC3. While absolute loading values are used to plot, the closed and open circles represent (+) and (–) loading values, respectively. P < 0.05 is the significance level for all tests

Article Snippet: NOD and Wistar rats (Charles River, USA) were maintained under standard housing conditions and fed with commercial pelleted chow (Harlan, USA) until ready for the study at age of 24 weeks.

Techniques: Expressing

Journal: Biology of Sex Differences

Article Title: Sexual dimorphism in myocardial acylcarnitine and triglyceride metabolism

doi: 10.1186/s13293-016-0077-7

Figure Lengend Snippet: Sex differences in NEFA homeostasis. a Profile of NEFA in cardiac tissue, as measured by LC-MS/MS, presented as fold change between NOD and Wistar rats by sex. An asterisk (*) indicates that the fold change is statistically significant. A section sign (§) indicates that the ANOVA interaction term is significant, and therefore, the fold changes in males and females also are. A dagger (†) indicates that in the ANOVA model, the overall sex difference is statistically significant. b Distribution of enriched and depleted of NEFA moities. The asterisk (*) indicates that the distribution is significantly different than a binomial distribution with mean = 0.5. The dagger (†) indicates that the distribution in males and females differ significantly. c , d The distribution of FAs based on their chain-length and degree of saturation, respectively. Data is presented as mean ± SEM ( N = 6). An asterisk (*) indicates that the difference in adjacent bars is statistically significant. A section sign (§) indicates that the ANOVA interaction term is significant. P < 0.05 is the significance level for all tests

Article Snippet: NOD and Wistar rats (Charles River, USA) were maintained under standard housing conditions and fed with commercial pelleted chow (Harlan, USA) until ready for the study at age of 24 weeks.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Biology of Sex Differences

Article Title: Sexual dimorphism in myocardial acylcarnitine and triglyceride metabolism

doi: 10.1186/s13293-016-0077-7

Figure Lengend Snippet: Sex differences in TG homeostasis. a Profile of TG species in cardiac tissue, as measured by LC-MS/MS, presented as fold change between NOD and Wistar rats by sex. An asterisk (*) indicates that the fold change is statistically significant. A section sign (§) indicates that the ANOVA interaction term is significant, and therefore, the fold changes in males and females are also significant. A dagger (†) indicates that in the ANOVA model, the overall sex difference is statistically significant. b Distribution of enriched and depleted of TG moities. The asterisk (*) indicates that the distribution is significantly different than a binomial distribution with mean = 0.5. The dagger (†) indicates that the distribution in males and females differ significantly. c The total amount of TG present in the cardiac tissue as measured by spectrophotometric analysis, mean ± SEM ( N = 6). P < 0.05 is the significance level for all tests

Article Snippet: NOD and Wistar rats (Charles River, USA) were maintained under standard housing conditions and fed with commercial pelleted chow (Harlan, USA) until ready for the study at age of 24 weeks.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Biology of Sex Differences

Article Title: Sexual dimorphism in myocardial acylcarnitine and triglyceride metabolism

doi: 10.1186/s13293-016-0077-7

Figure Lengend Snippet: Sex differences in AC homeostasis. a Profile of AC in cardiac tissue, as measured by LC-MS/MS, presented as fold change between NOD and Wistar rats by sex. An asterisk (*) indicates that the fold change is statistically significant. A dagger (†) indicates that in the ANOVA model, the overall sex difference is statistically significant. b Net ACs level in the tissue, identified by summing up individual AC species. The dagger (†) indicates that in the ANOVA model the overall sex difference is statistically significant. c Distribution of enriched and depleted of AC moities. The asterisk (*) indicates that the distribution is significantly different than a binomial distribution with mean = 0.5. The dagger (†) indicates that the distribution in males and females differ significantly. d Pooled distribution of ACs based on their chain length. e Pooled distribution of ACs based degree of saturation. Data in d and e are presented as mean ± SEM ( N = 6). The asterisk (*) indicates that comparison between adjacent bars is significant. The section sign (§) indicates that the ANOVA interaction term is significant. A dagger (†) indicates that in the ANOVA model, the overall sex difference is statistically significant

Article Snippet: NOD and Wistar rats (Charles River, USA) were maintained under standard housing conditions and fed with commercial pelleted chow (Harlan, USA) until ready for the study at age of 24 weeks.

Techniques: Liquid Chromatography with Mass Spectroscopy, Comparison